LABORATORY OF MOLECULAR MECHANISMS OF CELL METABOLISM REGULATION

I. Investigation of molecular mechanisms of phospholipases regulation in plant cells.

II. Study of physiologically active substances action on cereal plant cells metabolism: Molecular mechanisms of phospholipases and phytohormones crosstalk in plant cells under normal and stress conditions.

III. • Analysis of recognition mechanisms of natural and synthetic plant growth regulators to apply molecular organization of cell sensor systems in biotechnology and agriculture.

IV. Study of phospholipases mechanism functioning during adaptation of plant cells to stress conditions.

V.Brassinosteroids effect on alternative pathway of electron transport in mitochondria.

VI. Study of phospholipases and brassinosteroids on formation of PA, DAG and respiration processes in mitochondria.

LEGEND:

Wu, G., Wang, X., Li, X., Kamiya, Y., Otegui, M.S., Chory, J., 2011. Methylation of a phosphatase specifies dephosphorylation and degradation of activated brassinosteroid receptors. Sci. Signal. 4, ra29.
Tang, W., Yuan, M., Wang, R., Yang, Y., Wang, C., Oses-Prieto, J.A., Kim, T.-W., Zhou, H.-W., Deng, Z., Gampala, S.S., Gendron, J.M., Jonassen, E.M., Lillo, C., DeLong, A., Burlingame, A.L., Sun, Y., Wang, Z.-Y., 2011. PP2A activates brassinosteroidresponsive gene expression and plant growth by dephosphorylating BZR1. Nat. Cell Biol. 13, 124–131.
Samakovli, D., et al., Brassinosteroid nuclear signaling recruits HSP90 activity. New Phytologist, 2014: p. n/a-n/a.
He, J.X., et al., The GSK3-like kinase BIN2 phosphorylates and destabilizes BZR1, a positive regulator of the brassinosteroid signaling pathway in Arabidopsis. Proceedings of the National Academy of Sciences of the United States of America, 2002. 99(15): p. 10185- 10190
Zhao, J., et al., Two putative BIN2 substrates are nuclear components of brassinosteroid signaling. Plant Physiology, 2002. 130(3): p. 1221-1229.
The CDG1 Kinase Mediates Brassinosteroid Signal Transduction from BRI1 Receptor Kinase to BSU1 Phosphatase and GSK3-like Kinase BIN2. Molecular Cell, 2011. 43(4): p. 561-571.
Ryu, H., et al., Predominant actions of cytosolic BSU1 and nuclear BIN2 regulate subcellular localization of bes1 in brassinosteroid signaling. Molecules and Cells, 2010. 29(3): p. 291-296.
Ryu, H., et al., Nucleocytoplasmic Shuttling of BZR1 Mediated by Phosphorylation Is Essential in Arabidopsis Brassinosteroid Signaling. Plant Cell, 2007. 19(9): p. 2749- 2762.
Ryu, H., et al., Phosphorylation dependent nucleocytoplasmic shuttling of BES1 is a key regulatory event in brassinosteroid signaling. Molecules and Cells, 2010. 29(3): p. 283-290.
Ryu, H., et al., Predominant actions of cytosolic BSU1 and nuclear BIN2 regulate subcellular localization of bes1 in brassinosteroid signaling. Molecules and Cells, 2010. 29(3): p. 291-296
Kim, T.-W., et al., The CDG1 Kinase Mediates Brassinosteroid Signal Transduction from BRI1 Receptor Kinase to BSU1 Phosphatase and GSK3-like Kinase BIN2. Molecular Cell, 2011. 43(4): p. 561-571.
Wimalasekera, R., et al., Plant Phosphatidylcholine-Hydrolyzing Phospholipases C NPC3 and NPC4 with Roles in Root Development and Brassinolide Signaling in Arabidopsis thaliana. Molecular Plant, 2010. 3(3): p. 610-625.
Pokotylo, I.V., Kretynin, S.V., Khripach, V.A., Ruelland, E., Blume, Y.B., Kravets, V.S., 2014b. Influence of 24-epibrassinolide on lipid signalling and metabolism in Brassica napus. Plant Growth Regul. 73, 1–9.
Gao, H.-B., Chu, Y.-J., Xue, H.-W., 2013. Phosphatidic acid (PA) binds PP2AA1 to regulate PP2A activity and PIN1 polar localization. Mol. Plant 6, 1692–1702.
Wu, P., Gao, H.-B., Zhang, L.-L., Xue, H.-W., Lin, W.-H., 2014. Phosphatidic acid regulates BZR1 activity and brassinosteroid signal of Arabidopsis. Mol. Plant 7, 445–447.
Yu, X., et al., A brassinosteroid transcriptional network revealed by genome-wide identification of BESI target genes in Arabidopsis thaliana. Plant Journal, 2011. 65(4): p. 634-646.
Lisso, J., et al., Identification of brassinosteroid-related genes by means of transcript co-response analyses. Nucleic Acids Research, 2005. 33(8): p. 2685-2696.
Wang, C., et al., Identification of BZR1-interacting proteins as potential components of the brassinosteroid signaling pathway in Arabidopsis through tandem affinity purification. Molecular & Cellular Proteomics, 2013.
Nam, K.H. and J. Li, BRI1/BAK1, a receptor kinase pair mediating brassinosteroid signaling. Cell, 2002. 110(2): p. 203-212.
Sadura, I., Pociecha, E., Dziurka, M. et al. Mutations in the HvDWARF, HvCPD and HvBRI1 Genes-Involved in Brassinosteroid Biosynthesis/Signalling: Altered Photosynthetic Efficiency, Hormonal Homeostasis and Tolerance to High/Low Temperatures in Barley. J Plant Growth Regul 38, 1062–1081 (2019). https://doi.org/10.1007/s00344-019-09914-z
Anna Janeczko, Brassinosteroids in Cereals – Presence, Physiological Activity and Practical Aspects, In,: S. Hayat et al. (eds.), Brassinosteroids: Plant Growth and Development 2019, Chapter 3, p. 59-88. https://doi.org/10.1007/978-981-13-6058-9_3
Damian Gruszka Genetic and Molecular Bases of Brassinosteroid Metabolism and Interactions with Other Phytohormones, In,: S. Hayat et al. (eds.), Brassinosteroids: Plant Growth and Development 2019, Chapter 8, 219-249. https://doi.org/10.1007/978-981-13-6058-9_8

VII.Brassinosteroids and auxin singaling

LEGEND:
IAA – auxin, TIR1-SCF - TRANSPORT INHIBITOR RESPONSE 1- Skp1-Cullin-F-box, DWF4 – BRs bisosynthesis gene, BRs – brassinosteroids, BRI1 - BRASSINOSTEROID INSENSITIVE 1, BAK1 - BRI1-ASSOCIATED RECEPTOR KINASE 1, BIN2 - BR-INSENSITIVE2, BES1 - bri1-EMS-SUPRESSOR 1, BZR1 - BRASSINAZOLE RESISTANT 1, BSU1 - bri1-SUPRESSOR1, ARF – auxin response factor, PP2A – protein phosphatase 2A.